亚精胺对淹水胁迫下玉米幼苗根系抗氧化代谢调控
Regulation of Spermidine on Antioxidant Metabolism of Maize Seedling Roots under Waterlogging Stress
Abstract: In order to explore the regulation mechanism of spermidine on antioxidant metabolism of maize seedling roots under waterlogging stress, taking Zhengdan 958 (weaker anti -flood) and Xundan 22 (stronger anti-flood) as materials, this paper was carried out to study the effects of exogenous spermidine(Spd) on the activity of antioxidant enzymes and the content of metabolites of maize seedlings under waterlogging stress. Results are as follows: the activities of catalase (CAT), superoxide dismutase(SOD), ascorbic acid oxidase (APX) , peroxidase (POD) and the content of metabolites of maize seedling roots had no obvious change under normal condition; under waterlogging stress, the activities of CAT, SOD, APX, and POD decreased and superoxide anion (O2-) generation rate increased . However, after treatment with spermidine, the activities of CAT, SOD, APX and POD increased and superoxide anion generation rate decreased in Maize seedling roots. The results showed that Spd could relieve the injury of waterlogging stress on maize seedlings via increasing the activity of antioxidase of maize seedling root, and promote the growth of maize seedlings.
Keywords: Spermidine; Maize seedlings; Antioxidant metabolism; Waterlogging stress
目 录
摘 要： 1
引言 2
1.材料与方法 2
1.1供试材料 2
1.2材料处理 3
1.3 过氧化氢酶（CAT）活性的测定 3
1.4超氧化物岐化酶（SOD）活性的测定 4
1.5过氧化物酶（POD）活性的测定 5
1.6抗坏血酸氧酶（APX）活性的测定 6
1.7超氧阴离子O2-产生速率的测定 6
2. 结果与分析 8
2.1 涝胁迫对玉米幼苗CAT活性的影响 8
2.2 涝胁迫对玉米幼苗SOD活性的影响 8
2.3 涝胁迫对玉米幼苗POD活性的影响 9
2.4 涝胁迫对玉米幼苗APX活性的影响 10
2.5 涝胁迫对玉米幼苗组织O2-产生速率的影响 10
3. 结论与讨论 11
参考文献 12
致谢 14
亚精胺对淹水胁迫下玉米幼苗根系抗氧化代谢调控引言
玉米是我国重要的粮食、饲料及经济作物之一。长期以来，我国农产品总量基本上处于短缺状态，为了解决众多人口的吃饭问题，玉米一直被作为一种单纯的粮食作物，玉米研究也以增产为主要目标[1]。
渍涝是农业生产中的严重问题之一。根据联合国粮农组织的报告和国际土壤学协会绘制的世界土壤图估计，全球容易受到淹水危害的耕地面积约占总耕地面积的12％，共导致作物减产20％左右[2]，中国发生渍涝的土地面积约占总面积的2/3，严重制约着中国的粮食生产和发展[3]。研究表明，不同生长期、不同程度的涝害, 均会导致玉米产量的下降[4]。在淹水条件下，淹水使玉米根部缺氧, 无氧呼吸增强, 土壤大量有害物质积累, 根系环境恶化, 导致矿质离子和有益微量元素的吸收大大减少, 严重时会导致根系腐烂, 整株枯死[5]。
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