摘要近年来，随着影像学的发展,医学界对治疗成人股骨头坏死的研究又有了新的方法，现在应用骨髓间充质干细胞治疗成人股骨头坏死也有了新突破。骨髓间充质干细胞是存在于骨髓组织中的一类成体干细胞，来源于发育早期的中胚层和外胚层。利用骨髓间充质干细胞分化的多项潜能，来改善股骨头坏死区域的血液循环状态，促进病灶部位的血管的再生，以达到早期彻底治疗股骨头坏死的目的。骨髓间充质干细胞跨系、甚至跨胚层横向分化的可塑性及其强大的分化潜能使得它可为组织修复提供可能性。但有报道发现干细胞植入与传统髓心减压法疗效无显著差异，其原因可能在单纯干细胞的植入缺乏良好的骨支架材料。Bio-oss 骨粉是一种自体骨替代物质及骨组织再生支架，具有良好的生物相容性。也是一种很有前途的股骨头坏死的骨支架材料。实验通过原代取材、传代培养、共培养及诱导分化、microRNA提取及检测等，对Bio-oss 与骨髓间充质干细胞BMSCs共培养做相关基因分析。实验结果显示：bio-oss骨粉与细胞共培养后 miRNA-214 的表达上调。因此后续实验应用agomiRNA-214(miRNA-214 激动剂) 或antagomiRNA-214(miRNA-214 拮抗剂) 处理细胞，再与Bio-oss 骨 粉共培养，在动物模型体内试验考察 Bio-oss 骨粉联合处理后的细胞移植对骨坏死组织的修复效果。76275

In recent years, with the development of imaging, medical treatment of adult femoral head necrosis has been a new method, and now the application of bone marrow mesenchymal stem cells in the treatment of adult femoral head necrosis has also been a new breakthrough。 Bone marrow mesenchymal stem cells are present in bone marrow tissue in a kind of adult stem cells, derived from the development of the early mesoderm and ectoderm。 The bone marrow mesenchymal stem cell differentiation in a number of potential and to improve the femoral head necrosis area of blood circulation, promote the regeneration of vascular lesion, to achieve early thorough treatment stock bone necrosis to。 The plasticity of bone marrow mesenchymal stem cells and the lateral differentiation of mesenchymal stem cells, and even the differentiation potential of bone marrow mesenchymal stem cells and the differentiation potential of bone marrow mesenchymal stem cells can be provided for tissue repair。 But it is reported that there is no significant difference in the effect of stem cell implantation and traditional marrow decompression。 The reason may be that there is a lack of good bone scaffold materials in Dan Chungan cells。 Bio-oss is a kind of autogenous bone bone substitute materials and bone tissue regeneration scaffold has good biocompatibility。 It is also a promising bone scaffold material for femoral head necrosis。 Through primary culture, subculture, co culture, induction and differentiation, microRNA extraction and detection, Bio-oss and bone marrow mesenchymal stem cells were analyzed by BMSCs co culture。 Experimental results show that the up-regulated expression of miRNA-214 after co cultured Bio-Oss cells and bone。 The subsequent experimental application agomiRNA-214 (mirna-214had agonist) or antagomiRNA-214 (mirna-214had antagonist) treatment of cells, and co cultured with bio OSS bone powder, in vivo animal model testing study bio OSS combined treatment after cell transplantation of bone necrosis tissue repair effect。

毕业论文关键词：骨髓间充质干细胞；成骨细胞；股骨头坏死

Keyword: BMSC； osteoblast； femoral head necrosis

目    录

1、引言…4

2、材料与方法4

2。1、材料… 4

2。2、主要试剂… 4

2。3、骨髓间充质干细胞的原代取材 5

      2。3。1、直接全骨髓培养法5

      2。3。2、骨髓间充质干细胞的传代培养…5