摘要：本实验利用山梨醇和硼酸在一定条件下进行反应，配制成浓度为80mg/L的络合态硼肥—山梨醇硼（SB）。以大豆幼苗为实验材料，以无机硼酸（BA）为对照，在大豆幼苗生长至三叶一心期时，对其进行SB和BA的叶面喷施处理。喷施三至五个周期后，对大豆幼苗叶片进行一系列生理指标的测试，以研究络合态硼肥对大豆幼苗光合生理的影响。结果显示，区别于传统的无机硼酸，利用叶面喷施方法的络合态硼肥能显著提高大豆幼苗的各项相关生理指标，包括叶面的叶绿素荧光参数、叶绿素含量、光合作用速率等生理指标。78705

毕业论文关键词：络合态硼肥，大豆幼苗，叶绿素荧光参数，叶绿素含量，光合作用速率 

Abstract: Sorbitol and boric acid were used for a special reaction under certain conditions in this experiment, in order to compound 80 mg/L of sorbitol-boric acid complexes (SB) which were also named sorbitol boron (SB)。 Using boric acid (BA) as control, soybean seedlings with three leaves were employed as experimental material which were treated by foliar-spraying SB and BA respectively。 After being sprayed about 3 to 5 cycles, the leaves of soybean seedlings were tested for a series of physiological indexes for the purpose of studying the effects of sorbitol-boric acid complexes on the photosynthetic physiology of soybean seedlings。 The results showed that foliar-spraying sorbitol-boric acid complexes could improve the variety of related physiological indexes of soybean seedlings, including chlorophyll fluorescence parameters, chlorophyll content and photosynthetic rate, compared with the traditional boric acid。 

Keywords: sorbitol-boric acid complexes, soybean seedlings, chlorophyll fluorescence parameters, chlorophyll content, photosynthetic rate

目  录

1  前言 4

1。1  硼对植物的生理功能 4

1。1。1  硼对植物营养器官的生理功能 4

1。1。2  硼对植物生殖器官的生理功能 4

1。2 硼在植物体内的运输机制 5

1。2。1  硼在木质部的运输 5

1。2。2  硼在韧皮部的运输 5

1。3  山梨醇对植物的生理功能 5

1。4  植物体内硼的高效吸收 6

1。5  检验植物光合作用能力的生理指标 6

1。5。1  叶绿素荧光参数 6

1。5。2  叶绿素含量 7

1。5。3  光合作用速率 7

2  材料与方法 7

2。1  实验材料、仪器与试剂 7

2。1。1  实验材料 7

2。1。2  实验仪器 8

2。1。3  实验试剂 8

2。2  实验方法 8

2。2。1  两种不同形态硼肥的配制 8

2。2。2  大豆幼苗的培养及处理 8

2。2。3  大豆叶片叶绿素荧光参数检测 9

2。2。4  大豆叶片叶绿素含量检测