摘要：本试验利用硼酸与山梨醇在一定条件下进行反应，配制成一类络合态山梨醇与硼酸复合物的新型有机硼肥。并以黄瓜幼苗为试验材料，采用浓度为80mg/L的硼肥对三叶一心期的黄瓜幼苗叶片进行叶面喷施的处理。喷施三至五个周期后，对黄瓜幼苗叶片叶绿素荧光参数、叶绿素含量及光合速率等部分光合生理指标进行检测，以传统的无机硼肥为对照，研究了络合态硼肥对黄瓜幼苗光合生理的影响。结果显示，与传统的无机硼肥相比较，叶面喷施络合态硼肥能显著提高黄瓜幼苗的各项相关光合生理指标。80011

毕业论文关键词：络合态硼肥，黄瓜幼苗，光合生理，叶绿素荧光参数，叶绿素含量，光合速率

Abstract: Boric acid and sorbitol were used for a special reaction under certain conditions in this experiment, in order to compound a new kind of sorbitol-boric acid complexes。 Cucumber seedlings were used as experimental material which were treated by foliar-spraying with 80 mg/L sorbitol-boric acid complexes when they grew into the period of three leaves。 After being sprayed about 3 to 5 cycles, compared with boric acid, the leaves of cucumber seedlings were tested for a part of physiological indexes such as chlorophyll fluorescence parameters, chlorophyll content and photosynthesis rate for the purpose of studying the effects of sorbitol-boric acid complexes on the photosynthetic physiology of cucumber seedlings。 The results showed that differed from traditional boric acid, using the method of foliar-spraying sorbitol-boric acid complexes could improve the variety of related physiological indexes of cucumber seedlings。

Keywords: sorbitol-boric acid complexes, cucumber seedlings, photosynthesis physiology, chlorophyll fluorescence parameters, chlorophyll content, photosynthesis rate

目  录

1 前言 6

1。1 硼对植物的生理功能 6

1。2 络合态硼肥对植物的生理功能 7

2 材料与方法 7

2。1 实验材料、仪器与试剂 7

2。1。1 实验材料 7

2。1。2 实验仪器 7

2。1。3 实验试剂 8

2。2 实验方法 8

2。2。1 黄瓜幼苗的培养及处理 8

2。2。2 黄瓜幼苗叶片叶绿素荧光参数检测 8

2。2。3 黄瓜幼苗叶片叶绿素含量检测 9

2。2。4 黄瓜幼苗叶片光合速率检测 9

3 实验结果分析 10

3。1 黄瓜幼苗叶片叶绿素荧光参数检测结果及分析 10

3。1。1 两种形态硼处理对黄瓜幼苗叶片Fo、Fm、Fv、Fo'、Fm′和Fv′的影响 10

3。1。2 两种形态硼处理对黄瓜幼苗叶片Fv/Fm、Fv/Fo、ΦPSⅡ和NPQ的影响 11

3。2 黄瓜幼苗叶片叶绿素含量检测结果及分析 12

3。3 黄瓜幼苗叶片光合速率检测结果及分析 13

结  论 15

参 考 文 献 16

致  谢 18

附  录