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摘要本实验采用痕量壳聚糖包裹的多壁碳纳米管（CS-MWCNTs）作为分散微固相萃取（DMSPE）的吸附剂，同时结合超高效液相色谱（UPLC）与四极杆飞行时间串联质谱分析测定菊花茶和菊花饮料中的酚类化合物。在本实验中，首次将壳聚糖-多壁碳纳米管作为固相微萃取的吸附剂。此外，本实验方法具有简便，快速，样本量小和易于操作的优点。同时，所有影响萃取效率的重要参数，如吸附剂，pH值，提取时间，洗脱溶剂的类型，均在分散微固相萃取实验中进行了研究和优化。在最佳提取条件下，基于信噪比为3，检测极限为0.22-16.19纳克每毫克，所测试样品获得了较为满意的回收率，回收率为89%-106%。实验结果表明，本实验方法可成功应用于测定复杂菊花样品中的绿原酸和黄酮类化合物的含量。44045
ABSTRACT
This report describes the use of trace-chitosan-wrapped multi-walled carbon nanotubes (CS-MWCNTs) as a sorbent material in dispersive micro solid-phase extraction (DMSPE), which was combined with ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry to analyze phenolic compounds in chrysanthemum tea and a chrysanthemum beverage. In this study, for the first time, CS-MWCNTs were used as a sorbent for this microextraction mode. Moreover, the proposed method exhibits the advantages of simplicity, rapidity, small sample amount and ease of operation. Furthermore, all of the important parameters that affect the extraction efficiency, such as the sorbent, pH, extraction time and type of elution solvent, were investigated and optimized in the DMSPE. Under the optimized extraction condition, the limit of detection, which was calculated based on a signal-to-noise ratio of 3, was 0.22–16.19 ng mL−1. Satisfactory recovery values of 89–106% were obtained for the tested samples. The results show that the developed method was successfully applied to determine the content of chlorogenic acid and flavonoids in complex chrysanthemum samples.
毕业论文关键词：菊花样品； 黄酮类化合物； 分散微固相萃取； 壳聚糖-多壁碳纳米管；
Keyword: Chrysanthemum sample； Flavonoids； DMSPE； CS-MWCNTs
目 录
第一章 引言 4
1.1 碳纳米管概论 5
1.2 微萃取概论 5
1.3 菊花概论 6
第二章 实验部分 7
2.1 仪器与药品 7
2.2 色谱条件 7
2.3 对照品溶液及分散液制备过程 8
2.3.1对照品制备过程 8
2.3.2壳聚糖分散液制备过程 8
2.3.3壳聚糖-多壁碳纳米管分散液制备过程 8
2.4 药材样品制备过程 8
2.4.1菊花药材 8
2.4.2 杭白菊茶 8
2.5 操作步骤 9
2.6 分散液浓度考察 9
2.7 壳聚糖浓度考察 10
2.8 提取时间考察 10
2.9 母液PH考察 11
2.10 洗脱种类考察 11
2.11 洗脱体积考察 12
2.12标准曲线绘制 12
2.13重复性考察