富氢水对油菜幼苗耐盐性的影响及其机理研究
关键词：富氢水；盐胁迫；油菜；缓解；机理
Study on the effect of hydrogen-rich water on salt tolerance of rape seedlings and its mechanism
Student majoring in Life Science and Technique Base Zenong Yu
Tutor Xiaoming Xu
Abstract: Soil salinization is a major agricultural and ecological problem in the world today. This project is based on Zheyou 51 as material and the effect of hydrogen-rich water on salt tolerance of its seedlings was studied. Through the observation of plant phenotype and biomass, it was found that hydrogen-rich water had a mitigating effect on the salt stress of rape seedlings, and the suitable hydrogen-rich water and salt concentration were selected for processing experiment. Experiments show that in the aspect of the content of proline and protein, relative water content and photosynthesis, hydrogen-rich water have shown its role in alleviating plant salt stress. Through the detection of photosynthetic pigment content, a variety of antioxidant enzyme activity, hydrogen peroxide content, malondialdehyde content, relative conductivity, The mechanism of hydrogen-rich water to relieve plant salt stress by protecting photosynthetic pigments, accelerating the removal of oxidized free radicals and protecting the stability of biofilm systems was explained.
Key words: hydrogen-rich water(HRW); salt stress; rape; mitigation; mechanisms
目 录
摘要 1
关键词 1
Abstract 1
Key words 1
引言 1
1 材料与方法 2
1.1 实验材料 2
1.1.1 植物材料 2
1.1.2 Hoagland培养液 2
1.1.3 富氢水培养液 2
1.1.4 盐培养液 2
1.2 实验方法 2
1.2.1 材料的萌发及培养 2
1.2.2 筛选富氢水浓度和盐浓度 2
1.2.3 富氢水和盐处理幼苗 3
1.2.4 生理生化数据的测定 3
1.3 数据处理 4
2 结果与分析 5
2.1 筛选实验 5
2.1.1 植物表型 5
2.1.2 生物量 5
2.2 处理实验 6
2.2.1 脯氨酸（Proline）含量 6
2.2.2 蛋白质（Protein）含量 6
2.2.3 相对含水量（RWC） 7
2.2.4 光合作用参数 8
2.2.5 光合色素含量 9
2.2.6 抗氧化酶活性 9
2.2.7 过氧化氢（H2O2）含量 12
2.2.8 丙二醛（MDA）含量 13
2.2.9 相对电导率（Relative conductivity）13
2.2.10 Na+、K+离子含量的测定14
3 讨论 15
致谢 15
参考文献 16
富氢水对油菜幼苗耐盐性的影响及其机理研究
盐渍化土壤是全球分布广泛的一种土壤，目前全世界大约有不同程度的盐渍土壤 3.8亿公顷，占耕地面积的10%。我国盐渍地约有2.0×107 hm2，其中次生盐渍化土壤有6.7×106 hm2，占全国土地面积的14%。土壤盐渍化造成的盐胁迫严重影响植物生长发育，已经成为农业、生态的一个突出问题。因此，对于植物抗盐的研究显得十分必要[1]。油菜（Brassica campestris L.），又叫油白菜，苦菜，十字花科、芸薹属植物，是我国重要的农业经济作物[2]。氢是自然界中最简单、最丰富的元素，氢气作为21世纪的新能源，有环保无污染的特点[3]。氢气是难溶气体，在水中的溶解度很小，我们把氢气在当前温度下在水中的饱和溶液称为富氢水（Hydrogen-rich water, HRW）。富氢水制作简单安全，价格低廉，且浓度很低的富氢水就有较明显的作用。有实验证明，富氢水是一种优秀抗氧化剂，对于植物受到的多种胁迫危害都有一定的缓解作用[4, 5]。若能证明富氢水对于盐胁迫下的油菜幼苗有缓解作用，则可应用于改善盐渍土油菜种植问题，帮助粮食增产。