摘    要 小鼠是哺乳动物研究实验中首选的模式生物。经过几代科学家的努力，培育基因打靶和转基因小鼠的工作变得稀松平常，目前可以获得在核酸水平上的突变品系。现今诸如此种基因组的修饰趋于精细化，在基因水平上标记不同的细胞群的学术研究已非常普遍。90422

    人类基因组计划完成之后，生命科学基础研究急需一种行之有效的基因分析方法。而基因敲除小鼠模型的应用，恰恰为研究基因相对应的功能提供了给力的支持。在此基础上，可通过基因打靶与基因捕获这两种技术验证基因在疾病发生中的作用，从而揭示疾病的发生机制，推动医药学研究的发展。

GFP能够在体内或体外进行实时监测。在GFP中有两种全新颜色的变体ECFP和EYFP。由于ECFP和EYFP的谱线轮廓具天壤之别且无任何重叠，因此这两种荧光蛋白可共同显色并用作报导基因，这对体内双标记或荧光能量转移分析等研究是非常理想的[1]。

    因此本文将通过GFP用于分析lhx3基因表达模型的标签小鼠的基因型鉴定，进而研究lhx3基因的功能

Abstract Mouse as the preferred model organism in the study of mammalian genetics。 After decades of efforts, cμLtivate gene targeting and transgenic mice have become very common, to the current technology can be obtained at the nucleic acid level of the mutant lines。 Now, such as the modification of the genome is becoming more and more precise, and it has become very common to mark different cell popμLations at the gene level。

After completion of human genome project, the basic research of life science in urgent need of the genetic analysis of a kind of effective method。 Application of gene knockout mice model, just provides the force of support for the study of gene function。 On this basis, through gene targeting and gene capture both techniques it can verify the role of genes in the disease, so as to reveal the happening of the disease mechanism and promote the development of medical science research

 GFP can be real-time monitoring in vivo or in vitro。 In GFP there are two different colors of the variant ECFP and EYFP。 Because the ECFP and EYFP spectral line profile distinct and there is no overlap, so the two fluorescent protein can be also used as report gene and common color, which the body double labeling or fluorescence energy transfer analysis and so on is very ideal。 

 Therefore, this paper will be used to analyze the identification of the gene expression model of lhx3 gene in mice by GFP。 It can study the function of lhx3 gene in futher。

毕业论文关键词：转基因老鼠； GFP引物； PCR扩增

Key words: transgenic mice； GFP primer； PCR amplification

目录

摘    要 2

Abstract 2

1 引言 5

1。1 基因简介 5

2 实验原理、材料源Q于W优E尔A论S文R网wwW.yOueRw.com 原文+QQ75201,8766 与方法 5

2。1 实验原理 5

2。2 实验材料 6

2。2。1 实验试剂 6

2。2。2 实验仪器 6

2。3 实验方法 7

2。3。1