KIF4基因对小鼠卵子成熟的分子调控机制
毕业论文关键词：KIF4a；纺锤体；卵母细胞成熟
Molecular regulation mechanism of KIF4 on mouse egg maturation
Abstract：During mammalian oocyte meiosis, the meiotic spindle from the central oocyte to subcortical localization is the first step for establishment of cortical polarity, which is essential for two consecutive rounds of unique asymmetric cell pisions. So the spindle is necessary in the process of asymmetric meiosis, which ensures the stability of the genetic material in the oocyte and is ready for fertilization. KIF4A is one of the newly discovered kinesin family members. It is an important protein founded in chromosome agglutination and sequestration. Transfection of PRCI protein into the central region of mitosis is involved in microtubule binding at the later stage of mitosis. However, the mechanism of KIF4 in the oocyte of mouse is not clear. Therefore, in order to determine the effect of KIF4a on the maturation process of oocyte of mouse , we used immunofluorescence to detect the expression of KIF4a in the vicinity of the spindle in the oocytes. And the laser confocal results showed that KIF4a activity was inhibited will lead to abnormalities of spindle and chromosome ; Through observe the oocytes which injected by KIF4a antibody by use microscope. We found KIF4a activity will be inhibited after the oocyte caused by the polar body cannot be properly discharged. Thus, KIF4a may affect the meiotic pision of oocytes by affecting the formation of the spindle.
Key words: KIF4a；spindle；oocyte meiosis
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法2
1.1材料 2
1.1.1实验动物2
1.1.2抗体和试剂2
1.1.3仪器设备2
1.2方法 3
1.2.1卵母细胞的收集和体外培养3
1.2.2 显微注射KIF4a抑制剂处理卵母细胞3
1.2.3免疫荧光染色和激光共聚焦分析3
1.2.4统计分析3
2结果与分析3
2.1 KIF4a的定位与表达3
2.2 KIF4a影响小鼠卵母细胞成熟过程中的极体排出4
2.3 KIF4a影响纺锤体在卵母细胞减数分裂过程中的形成5
3讨论 6
致谢7
参考文献7
KIF4基因对小鼠卵子成熟的分子调控机制
引 言
随着现代畜牧业的的进步，使得我们对动物繁育和生殖调控技术的需求越来越高。体外受精技术越来越成熟，我们对卵母细胞发育机制的了解也越发的深入，解决了许多动物繁育甚至人类生殖障碍方面的问题。但试管动物以及试管婴儿的出生率依旧无法与自然分娩的相比，大约只有20% [1]。而试管动物出生率的决定因素之一即是卵母细胞质量的好坏和其成熟分裂的程度。因此卵母细胞成熟机制的研究对于畜牧发展和辅助生殖有着十分重要的意义。