利用酵母验证水稻基因的耐镉性
毕业论文关键词：水稻基因；耐镉性；酵母
Verification of cadmium tolerance in rice gene with yeast
Abstract：With the rapid development of molecular biology in recent decades,gene amplification with molecular tools becomes more efficient , prokaryote and eukaryote expression system is far more better.They play an increasingly important role in the field of biological research. At the same time, along with the development of hundreds years of industrialization,the serious problem that all human beings need to face is heavy metal pollution. Cadmium accumulation in main food, such as rice , affects billions of people in the worldwide . It has become an important project to explore the mechanism of plant tolerance to heavy metals by using molecular biology methods. In this paper, we use the method of molecular biology to study the genes which is possibly related to cadmium tolerance，such as ATP synthase，photosystem I assembly protein ycf4 and abscisic stress ripening protein.They are transformed into the yeast and tested by medium containing cadmium.The results showed that the expression of four genes could make cadmium sensitive strain get more toleranceto cadmium, and the mechanism needed to be further explored.
Key words: rice gene;cadmium tolerance;yeast
目 录
摘要1
关键词1
Abstract1
Key words1
引言1
1材料与方法2
1.1 实验材料2
1.2 实验方法2
1.2.1 目的基因的扩增与回收2
1.2.2 质粒的构建3
1.2.3 酵母的转化与培养4
2 结果与分析5
2.1酵母转化子培养结果5
2.2酵母异源功能互补实验6
3 讨论 7
致谢9
参考文献10
利用酵母验证水稻基因的耐镉性引言：镉作为一种重金属元素，在自然界含量稀少，分布广而散,各种生物、土壤和水体中都含有微量镉，在环境中容易累积,且不能降解。镉在植株体内的作用是缓慢增加的,细胞还没有表现出明显的受毒害的症状时，实际上植株体内已经积累了大量的镉。粗放的工业化向环境中排出了大量未经处理的污染物，用含过量重金属的污水灌溉、将重金属污染区域辟为农田以及肥料的不当施用等导致稻田受重金属污染的问题更加严重[1]。水稻是中国种植量最大的粮食作物之一，也是玉米、小麦等世界上最主要的粮食作物之一，地球上有一半的人把稻米当做日常主食[2]。土壤中过高含量的镉，不仅会被水稻富集，在其各组织细胞中积累, 影响植株的生长，导致亩产量降低；还会通过生物链最终富集到人体内，且无法被代谢排出，造成慢性毒害[3]。探索水稻耐镉的机理，研究水稻植株中关于镉耐受的基因表达,源^自#优尔^文~论`文]网[www.youerw.com，有助于定位水稻基因组中指导转录相关蛋白的基因；进行转基因研究，培育出耐镉且不易富集的品种；可以改善现有植株中果实重金属积累过多、危害人体健康的状况。