黄瓜CsAFB基因影响拟南芥盐胁迫耐受性的研究
毕业论文关键词：盐胁迫；转基因拟南芥；CsAFB；耐盐机制
Abstract
Auxin receptors participate in transcriptional regulation under salt stress, which influence the salt stress tolerance in Arabidopsis. We had cloned the auxin receptor homologous genes CsAFB (auxin signaling F box protein) in cucumber (Cucumis sativus L.) in our previous work, whose function had not been reported yet. The transgenic CsAFB over-expressed Arabidopsis were obtained successfully before，so we used homozygous transgenic plants as materials to explore the potential influence of CsAFB in salt stress tolerance and study its mechanism in Arabidopsis. In this work, both transgenic CsAFB and wild-type plants were treated with 300 mM NaCl, germination rate of seeds were counted, and the growth of seedings were observed and measured.
The main results are as follows: under 300 mM NaCl, transgenic plants had an enhanced seed germination percentage and rate, increased numbers of lateral roots, and decreased inhibition of chlorophyll synthesis. The SOD activity increased as the content of MDA decreased in adult plant leaves in transgenic CsAFB plants. This work illustrated that transgene of CsAFB enhanced salt stress tolerance in arabidopsis thaliana. which functioned similarly as AtTIR1. This work also contributes to the further study of their functions and mechanisms in salt stress tolerance in cucumber.
Keywords：Salt stress; Transgenic Arabidopsis; CsAFB; Salt tolerance mechanism
目 录
引言 4
1 黄瓜CsAFB基因影响拟南芥盐胁迫耐受性 6
1.1 材料与方法 6
1.1.1 实验材料 6
1.1.2 实验主要试剂和仪器 6
1.1.3 盐胁迫下种子萌发率统计 6
1.1.4 盐胁迫下幼苗根的生长情况统计 7
1.1.5 盐胁迫下土培苗生长状态统计 8
1.2 实验结果 8
1.2.1 黄瓜CsAFB基因影响种子在处理下的萌发率 8
1.2.2 黄瓜CsAFB基因影响盐处理下根的生长 9
1.2.3 黄瓜CsAFB基因影响植株在盐处理下的生长状态 10
1.3 讨论 11
2 黄瓜CsAFB基因影响拟南芥耐受性的机理研究 12
2.1 材料与方法 12
2.1.1 材料 12
2.1.2 主要试剂和主要使用仪器 12
2.1.3 SOD酶活性测定 12
2.1.4 丙二醛含量测定 13