不同的碳源对里氏木霉产纤维素酶的影响的研究
摘 要：随着能源危机的加剧，天然纤维质原料酶解生产燃料酒精成为当前研究的热点。利用微生物产生的纤维素酶来分解和转化纤维素是纤维素利用的有效途径，对解决环境污染、食品短缺、能源危机和人类社会的可持续发展具有重大的现实意义。本实验探究不同的碳源对里氏木霉产纤维素酶的影响并研究适合里氏木霉产纤维素酶的最佳碳源。结果显示，葡萄糖抑制里氏木霉产纤维素酶，本实验中用于对照。而以1%的微晶纤维素为碳源时对里氏木霉产纤维素酶的诱导效果最好，产酶历程中，最大的滤纸酶活为0.36U，比5%葡萄糖作碳源大0.34U；以3%的CMC为碳源时有较好的产酶诱导作用，滤纸酶活最大为0.25U，比5%葡萄糖作碳源大0.23U；其次是以3%的秸秆为碳源，滤纸酶最大达到0.18U，比5%葡萄糖作碳源大0.16U。65736
毕业论文关键词：里氏木霉，纤维素酶，微晶纤维素，CMC，秸秆
Abstract: Currently, the research of using the natural fiber raw material to produce fuel alcohol has been paid more and more attention because of the aggravating of energy crisis. Using cellulase produced by microorganisms to decompose and convert of cellulose is an effective way of cellulose utilization. This is of great practical significance in solving the environment pollution, food shortage, energy crisis and the sustainable development of human society. The thesis is to explore the effects of different carbon sources in the cellulase production by Trichoderma reesei. The results show that the glucose inhibits cellulase production by T. reesei and used as the control group in this thesis. With microcrystalline cellulose as the carbon source, 1% microcrystalline cellulose gives the best cellulase activity, the highest filter paper activity reaches 0.36U. The cellulase activity increased 0.34U when compared with 5% glucose as carbon source. CMC is also a good inducer for cellulase production, the highest filter paper activity reaches 0.25U. Secondly, with 3% of straw as the carbon source, the highest filter paper activity reaches 0.18U, compared with 5% glucose as carbon source have increased 0.16U.
Keywords: Trichoderma reesei, Cellulase, Microcrystalline cellulose，CMC，Straw
目 录
1 前言 4
2 材料与方法 4
2.1 材料 4
2.1.1 菌种 4
2.1.2 培养基 4
2.1.3 试剂 5
2.2 主要仪器设备 5
2.3 实验方法 6
2.3.1 菌种培养 6
2.3.2 纤维素酶粗酶液制备 6
2.3.3 葡萄糖标准曲线的制备 6
2.3.4 滤纸酶活的测定 6
2.3.5 滤纸酶活的计算方法 7
3 结果与讨论 7
3.1 结果 7
3.1.1 葡萄糖标准曲线的绘制 7
3.1.2 不同浓度的葡萄糖对里氏木霉产纤维素酶的影响 8
3.1.3 不同浓度的微晶纤维素对里氏木霉产纤维素酶的影响 9
3.1.4 不同浓度的CMC对里氏木霉产纤维素酶的影响 10
3.1.5 不同浓度的秸秆对里氏木霉产纤维素酶的影响