摘要：本论文以植物乳杆菌作为菌种，通过工艺优化进行高密度培养，对获得的菌体进行冷冻干燥处理，并优化冻干工艺。首先以pH和活菌数为指标，通过单因素对乳酸菌发酵过程进行优化，最后通过响应面实验，得出最适培养温度为37℃，初始pH6。30，添加2%酵母抽提物、果糖、麦精，0。7%番茄汁以及接入1。1%乳酸菌培养24小时，活菌数为4。4×109CFU/mL。通过进行冷冻干燥的工艺优化，最终获得最适冻干保护剂配方：脱脂乳粉100g，乳糖100g，抗坏死血酸钠10g，谷氨酸钠10g。1:10比例与菌泥混合，得到的冻干菌粉活菌数为4。3×1011CFU/g。81291

毕业论文关键词：酵素；高密度培养，乳酸菌；活菌数；冷冻干燥 

Study on high density culture of lactic acid bacteria in Biozyme and optimization of freeze-drying process

Abstract: In this paper, Lactobacillus plantarum was used as a strain, and high density culture was optimized by process optimization。 The obtained cells were freeze-dried and optimized。 Firstly, the fermentation process of lactic acid bacteria was optimized by single factor, and the optimum culture conditions were as follows: 37 ℃ and pH6。30 were used to extract 2% yeast extract。 , 0。7% tomato juice and 1。1% lactic acid bacteria were cultured for 24 hours, the viable count was 4。4 × 109CFU/mL。 The final freeze-dried protective agent formula was: degreased milk powder 100g, lactose 100g, sodium necroicide sodium 10g, sodium glutamate 10g 1L distilled water to dissolve and mix to 1:10 The ratio of bacteria and bacteria mixed, the freeze-dried bacteria powder viable count of 4。3 × 1011CFU/g。

Key words: Enzyme; high density culture, lactic acid bacteria; viable count; freeze drying

目录

1 绪论 1

1。1酵素的概念、功能及其产品形式 1

1。1。1酵素的概念及功能 1

1。1。2酵素的制备 2

1。2制备酵素粉中的关键技术 3

1。2。1乳酸菌的高密度培养 3

1。2。2 乳酸菌的冷冻干燥 3

1。3本课题研究目的及意义 3

2  材料与方法 4

2。1  菌种、培养基与试剂以及配置方法 4

2。2  仪器与设备 5

2。3  实验方法 6

2。3。1菌体活化以及果汁的制备 6

2。3。2 pH计的校正及测定 6

2。3。3菌体密度的测定 6

2。4单因素实验 6

2。4。1最适培养时间 6

2。4。2最适pH 6

2。4。3最适碳源 7

2。4。4最适缓冲盐 7

2。4。5最适添加物 7

2。5 响应面的优化和验证 7

2。5。1 响应面方案设计 7

 2。5。2 回归模型的建立及方差分析 8

2。6 冷冻干燥工艺优化 8

2。6。1冷冻干燥的操作步骤 8

2。6。2 冻干保护剂的单因素实验