摘要: 本文4-羟基癸酸（盐）是由丙位癸内酯(γ-decalactone，GDL)进行逆向水解而制备。即以γ-癸内酯、水、氢氧化钠为原料，在100℃下利用皂化反应制备4-羟基癸酸钠，多次用氯化钙溶液进行复分解反应，经洗涤、抽滤、烘干得到纯度为99。4％的4-羟基癸酸钙，并用红外光谱仪、核磁共振色谱仪对产物的结构进行定性鉴定。传统认为生物转化制备GDL的代谢路径是蓖麻油酸通过4次β-氧化后形成4-羟基癸酸(4-HD)，其作为内酯重要前体物经环化酶形成GDL。但4-羟基癸酸的化学性质不稳定在常温中性和偏酸的环境下容易环化成GDL，所以通过制备4-羟基癸酸钙加在含磷酸缓冲液（把钙盐缓慢转化成）培养基中，加入酵母粉10g/L进行48h生物转化生成GDL的方法，并用气相色谱仪测出产物的浓度。对其转化条件优化后得到起始PH为7。 6、底物浓度为2。37g/L、转化时间为60h时4-羟基癸酸钙生物转化效率最高。77003

毕业论文关键词：4-羟基癸酸（盐）；γ-癸内酯；皂化反应；生物转化；红外光谱

Preparation and characterization of 4 - hydroxydecanoic acid (salt), an important precursor ofγ-decalactone

Abstract: 4-hydroxydecanoic acid (salt) is prepared by reverse hydrolysis of gamma-decalactone (GDL)。 A solution of sodium 4-hydroxydecanoate was prepared by saponification at 100 ° C using γ-decalactone, water and sodium hydroxide as raw materials。 The reaction was repeated with calcium chloride solution, washed, filtered and dried The purity of 99。4% calcium 4-hydroxydecanoate was obtained, and the structure of the product was qualitatively identified by infrared spectroscopy and nuclear magnetic resonance spectroscopy。 It is believed that the metabolic pathways of GDL for biotransformation are that ricinoleic acid forms 4-hydroxydecanoic acid (4-HD) by 4-fold β-oxidation, which forms GDL as cyclone as an important precursor of lactone。 But the chemical properties of 4-hydroxydecanoic acid instability in the normal temperature neutral and acidic environment easily ring into GDL, so by the preparation of calcium 4-hydroxydecanoate in pHospHate-containing buffer (the calcium salt slowly into) culture The addition of yeast extract 10g / L for 48h biotransformation to produce GDL method, and the gas chromatograpH measured product concentration。 The initial pH was 7。 6, the substrate concentration was 2。37 g / L, and the conversion time was 60 h, the conversion efficiency of 4 - hydroxydecanoate was the highest。

Keywords: 4 - hydroxydecanoic acid (salt)；γ-decalactone； Saponification reaction；Biotransformation；IR

目录

1。绪论 1

1。1 4-羟基癸酸（盐）与γ-内酯介绍 1

1。2 4-羟基癸酸（盐）与γ-癸内酯的制备 1

1。3 实验方法研究 2

1。4 本课题的目的及研究意义 3

2。材料与方法 4

2。1仪器与试剂 4

2。1。1仪器 4

2。1。2试剂 4

2。2 仪器与设备 5

2。3 实验方法 5

2。3。1 4-羟基癸酸钙的制备方法 5

2。3。2 4-羟基癸酸的表征 9

2。3。3 4-羟基癸酸钙生物转化成γ-癸内酯 10

2。3。4 γ-癸内酯的气相色谱分析 12

3。结果与讨论