摘要：柚子皮精油有独特的芳香，主要源于柚子皮中黄色部分的外皮中，精油不仅在柚子皮中所占含量少还难于提取。本实验使用蒸馏法，获得柚子皮精油，并研究采用最佳提取条件，使得在相同条件下柚子皮精油的提取率最高。同时采用气相和气质联用对精油进行了精油的成分测定，检测结果表明柚子皮精油中主要含有D-柠檬烯、β-月桂烯、吉马烯-D、圆柚酮、β-水芹烯等成分。对于获得的柚子皮精油产物，分别使用荧光法和分光光度法两种不同方法研究柚子皮精油含量对于自由基清除能力的影响，在荧光法中柚子皮精油相比同实验使用的Ve对自由基的清除能力相对较弱，此现象在分光法的DPPH检测中也有相应的体现，两种方法均表明柚子皮精油的抗氧化性能低于Ve。80559

毕业论文关键词：荧光法；分光光度法；柚子皮精油

The antioxidant properties of essential oil from shaddock peel were determined by fluorescence and spectrophotometry

Abstract:Grapefruit essential oil has a unique aroma, mainly from the skin of the yellow part of grapefruit skin, essential oils are not only less in grapefruit skin, but also difficult to extract。In this experiment, the essential oil of shaddock peel was obtained by distillation, and the optimum extraction conditions were studied。 Under the same conditions, the extraction rate of essential oil of shaddock peel was highest。 And the gas phase of temperament composition of essential oils Determination of essential oils, mainly containing D- limonene, myrcene, germacrene -D, 3, phellandrene, and grapefruit peel oil for product obtained, respectively using fluorescence spectrophotometry method and two different research methods of grapefruit essential oil content the effect of free radical scavenging capacity, the fluorescence of grapefruit peel essential oil with the same experimental use of Ve on free radical scavenging capacity is relatively weak, this phenomenon is also the embodiment of DPPH, optical linear upward trend ve greater than grapefruit peel oil, antioxidant properties of essential oils that grapefruit skin is less than Ve。

Keywords: fluorescence method; spectrophotometry； Pomelo peel essential oil

目 录

1。绪论 1

1。1文献综述 1

1。1。1柚子皮精油概述 1

1。1。2 柚子皮精油的应用和合成香料 3

1。1。3 精油的提取 5

1。1。4 柚子皮精油提取的最优化工艺条件 5

1。1。5 气质联用分析法 6

1。1。6 精油的抗氧化性能研究 6

1。1。7 精油的抗氧化性能检测 7

1。1。8 比重的测定 7

1。2 选题意义 8

1。3 实验方案 8

2 实验仪器与设备 8

2。1 实验试剂 8

2。2 实验设备 10

2。3 试剂配制 10

2。3。1 DPPH溶液的配制 10

2。3。2 羟基溶液的配制 10

3 实验内容 11

3。1 精油的提取 11

3。2 气相气质色谱分析 12

3。2。1 气相色谱分析