利用植物甾醇转化医药中间体ADD工艺的初步研究
毕业论文关键词：植物甾醇；分枝杆菌；ADD；雄烯二酮
Preliminary Study on the Process of Manufacture Medication Intermediate ADD Using Plant Steroid
Abstract: Androst -4- ene -3,17- dione(AD) and androsta -1,4- diene -3,17- dione (ADD) are the important intermediate of steroid hormones. Almost all of the steroid hormone drugs can use ADD as the starting material for the production. With the gradual depletion of diosgenin and other natural resources, the method of producing ADD by chemical synthesis system is no longer applicable. It is well-known that phytosterols are new suitable raw materials for microbial degradation to ADD because of low cost and easy availability. In recent years, studies on preparation of ADD by mycobacteria degradation of plant sterols system have been widely carried out. In this paper, a strain preserved in our laboratory was used as the starting strain and soybean sterols as substrate. The process on production of ADD was explored. The main work of this study was as follows: the choice of carbon source, nitrogensourceof medium, iron ion and its inhibitor, the culture temperature, and adding amount of substrate were investigated. Then the concentrations of carbon source, nitrogen source, time for adding substrate, inducer concentration were optimized by orthogonal test. Preliminary determination of the optimum carbon source of medium was glucose, and its concentration was 7.5 g/L, the optimum nitrogen source was ammonium dihydrogen phosphate, the concentration was 2 g/L, medium cultured with 0.005 g/L ammonium ferric citrate, supplementary iron ions for cell growth, and add 0.025 g/L of six hydrate cobalt chloride before the substrate was added, the most suitable culture temperature was 30 ℃, the optimum substrate adding amount was 2g/L, the optimum substrate addition time was 24h, using AD as the inducer in fermentation culture medium, inducer concentration was 0.2 g/L. At this time, the highest yield of ADD was 0.5g/L, the conversion rate reacheed 25%, fermentation time was 8 days.
KeyWords：phytosterol；Mycobacterium；androstenedione
目录
1 前言 1
1.1 植物甾醇概述 1
1.1.1 植物甾醇理化性质 1
1.1.2 植物甾醇的种类及功能 1
1.1.3 植物甾醇的应用现状 2
1.2 甾体激素药物的发展及现状 2
1.2.1 甾体激素药物概述 3
1.2.2 甾体激素类药物的应用现状 3
1.2.3 雄甾二烯二酮（ADD）的研究及应用 4