摘    要 随着人类社会的进步和科学的发展，越来越多的人开始重视神经性疾病对人类的危害，全球大约有3。5亿人患有抑郁症，呆症状况在发展中国家较为严重严重，中国每年发病率约24%，还有帕金森，癫痫等疑难杂症和无数神经性慢性病对我们的影响愈来愈严重。为了解决Lhx6基因在神经方面的作用，需要利用条件性基因敲除技术培育出相应的突变小鼠作为实验器材。90137

基因敲除技术是利用实验将模式动物中的某些基因去除，培育成转基因物种，因缺失某个基因，在观察模式生物的生理功能和行为时，便能知道此被敲除基因的功能。在生命科学，医学和人类健康研究中，许多发生的病理和生理的过程与一些动物的生命活动都有类似。而且不同的动物与动物间也有相类似的习性，所以一种动物生命活动可以参考另一种动物或人类。当一些生命活动的研究无法在人类身上进行试验，而在大型哺乳动物身上也难以操作取材时，参考一些能用于研究目标且小型易操作的动物物种是很好的选择，这种供于研究类动物就是模式动物。现如今，最佳的动物模型就是小鼠。

    因此本文将通过对lhx6条件性敲除小鼠而培育出的转基因小鼠基因表达模型的标签小鼠的基因型分析，从而分析lhx6基因对于神经的功能及影响。

毕业论文关键词：Lhx6基因，基因敲除技术， 神经系统， PCR扩增技术，

Abstract   With the progress of human society and the development of science, more and more people began to pay attention to the harm of neurological diseases to humans。 About 350 million people in the world suffer from depression, and the situation is more serious in developing countries。 The annual incidence of about 24%, as well as Parkinson, epilepsy and other incurable diseases and numerous neurological chronic diseases on our impact more and more serious。 In order to solve the neurological role of Lhx6 gene, it is necessary to use the conditional gene knockout technique to cultivate the corresponding mutant mice as experimental equipment。

    Gene knockout technique is to use experiments to remove certain genes in the model animals and cultivate them into transgenic species。 Because of the lack of a gene, the function of the knocked gene can be known when observing the physiological function and behavior of the model organism。 In life science, medicine and human health research, many of the pathogenesis and physiological processes are similar to those of some animal life activities。 And different animals and animals also have similar habits, so an animal life activities can refer to another animal or human。 When some of the research of life activities can not be tested on human beings, and in large mammals are also difficult to manipulate the material, with reference to some can be used to study the target and small easy to operate animal species is a good choice, this for the study Animals are model animals。 Now, the best animal model is the mouse。 

    Therefore, the genotype analysis of tagged mice in the transgenic mouse gene expression model of lhx6 conditioned knockout mice was used to analyze the function and effect of lhx6 gene on nerve。

Key words: Lhx6 gene, gene knockout technique, nervous system, PCR amplification technique,

目录

第一章 引言 1

1。1背景介绍 1

1。1。1基因敲除 1

1。1。2实现基因敲除的多种原理和方法 1

1。1。3模式动物 1

1。1。4 Lhx6基因简介及应用