摘要【目的】实验通过研究HAND2基因在徐淮山羊胎羊各组织的表达差异性来了解HAND2基因的结构功能。【方法】 通过使用实时荧光定量 PCR 反应技术，对目的基因和内参基因的扩增进行比对，使用SPSS统计软件将数据进行处理制表，使用Origin75制作柱状显著差异图，便于分析各组之间的表达差异。采集山羊八个组织的组织块，即为心、肝、脾、肺、肾、肠、背、腿，提取组织块的总RNA，进行反转录，得到总的cDNA，实时荧光定量PCR扩增HAND2基因，整理数据并分析得出结论。【结果】HAND2基因在徐淮山羊各组之间的表达量各不相同，具有显著性差异，其中心肌表达量最高，背肌、腿肌的表达量相对较高，肝、脾、肺、肾的表达量较低，肠的表达量最低，其由低到高的顺序为心（1.0000）>背（0.4235）>腿（0.1222）>脾（0.0494）>肺（0.0461）>肾（0.0363）>肠（0.0137），说明HAND2基因可能对山羊肌肉组织生长发育有影响。71127

该论文有图5幅，表3个，参考文献11篇

毕业论文关键词：HAND2基因  定量PCR  表达差异  徐淮山羊

The Study on Different Expression of HAND2 Gene in Different Fetal Goat Organs

Abstract [Objective] The experiment in genetic research HAND2 Xuhuai goat of the organizations to understand the differences in the expression of structure and function HAND2 gene. [Method] By using real-time PCR reaction technique for gene and reference gene were amplified by comparison, using SPSS statistical software to process the data tabulation using Origin75 make a significant difference columnar FIG facilitate analysis between the groups the differential expression. Collecting tissue goat eight organizations, namely the heart, liver, spleen, lung, kidney, intestine, back, legs, total RNA was extracted tissue blocks, reverse transcription to obtain total cDNA, real-time PCR amplification HAND2 gene, organize and analyze data to draw conclusions. [Results] HAND2 gene expression between the groups Xuhuai goats vary, with significant difference among the expression of cardiac highest expression, back muscles, leg muscle is relatively high, liver, spleen, lung, kidney the expression levels are low, the lowest expression of the intestine, which consists of low to high order of heart (1.0000)> back (0.4235)> leg (0.1222)> spleen (0.0494)> lung (0.0461)> kidney (0.0363)> intestinal (0.0137), indicating HAND2 gene may affect the growth of muscle tissue goat.

Key words: HAND2 gene  quantitative PCR  expression differences  Xuhuai goat

目录

摘要 I

Abstract II

目录 III

图清单 Ⅳ

表清单 Ⅳ

1绪论 1

2 实验过程及方法 2

2.1实验过程 2

2.2实验材料 2

2.2.1实验样本的采集 2

2.2.2实验仪器与设备 2

2.2.3实验试剂 2

2.3实验步骤 3

2.3.1提取组织块的总RNA 3

2.3.2样品质量的检测 4

2.3.3反转录合成cDNA 4

2.3.4实时荧光定量PCR 4

2.3.5数据处理方法