湖羊ACTA1基因遗传多样性初步分析
摘要本实验利用PCR-SSCP和DNA测序技术对湖羊ACTA1基因的遗传特征做相关研究，来确定遗传多样性以及获得相应的分子遗传学信息，找到与体尺性状相关的DNA标记，通过对多态位点的测序分析，为湖羊的进一步选育提供理论依据。采集湖羊耳朵组织块，使用常规饱和苯酚-氯仿提取基因组DNA，测量并记录它的纯度和浓度，PCR进行扩增，琼脂糖凝胶电泳检测DNA扩增情况。对扩增情况好的进行PCR-SSCP检测，多态产物纯化测序，再进行序列分析以及基因型判定，最后数据分析。实验结果显示，湖羊ACTA1基因存在着一个多态位点P3，多态信息含量为0.314，属于中度多态，基因型包括AA和AB两种，基因型频率分别是0.383和0.617，其中AB为优势基因型，经过方差分析发现ACTA1基因的遗传多样性对于湖羊的胸围具有显著影响，而对于其它的体尺性状没有影响或影响不显著。71678
该论文有图7幅，表7个，参考文献16篇。
毕业论文关键词：湖羊 ACTA1基因 DNA测序 PCR-SSCP 多样性分析
Preliminary analysis on genetic persity of ACTA1 gene in Hu sheep
Abstract In this study, PCR-SSCP and DNA sequencing of the genetic characteristics of the Hu sheep ACTA1 genes do research to determine genetic persity, as well as to obtain the corresponding molecular genetic information to find and economy-related DNA markers by polymorphic sequencing site analysis, to provide a theoretical basis for further breeding Hu sheep . The Hu sheep ear tissue collected using conventional saturated phenol / chloroform extraction of genomic DNA, measure and record its purity and concentration, PCR amplification, agarose gel electrophoresis DNA amplification. Good for amplification were PCR-SSCP detection of polymorphic sequencing products were purified, and then sequenced and analyzed genotype determination, the final data analysis. Experimental results show that there is a hu sheep ACTA1 gene polymorphism loci P3, polymorphic information content was 0.314, which belongs to moderate polymorphisms, including genotypes AA and AB are two kinds of genotype frequencies were 0.383 and 0.617, wherein AB dominant genotype, through analysis of variance revealed that genetic persity ACTA1 gene for the Bust Hu sheep have a significant impact, and for other body size traits no effect or no significant effect.
Key words : hu sheep ACTA1 gene DNA sequencing PCR-SSCP persity analysis
目 录
摘要 I
Abstract II
目 录 III
图清单 Ⅴ
表清单 Ⅴ
1 引言 1
2 材料和方法 2
2.1 实验过程 2
2.2 实验材料 2
2.2.1实验样品来源 2
2.2.2实验器材设备 2
2.2.3实验试剂及药品 3
2.3 实验方法 3
2.3.1提取组织块的总DNA 3
2.3.2提取的总DNA的质量检测 4
2.3.3样品DNA标准纯化 4
2.3.4引物设计