摘要：以四川干辣椒为原料，通过选择使用六种不同的溶剂来对此干辣椒中红色素做提取实验，以此来选择出最优溶剂，并用薄板层析法和柱层析技术对所制备产物进行了组分成分的分析。实验采用索氏提取器提取辣椒红色素，考察了不同的溶剂提取效果以及不同的反应时间对产物产率的影响；对辣椒红色素进行减压蒸馏浓缩，进行脱辣处理，进行脱辣评定；实验又运用四种不同的展开剂来进行薄层分析，分析出最适的展开剂，利用柱层析法对辣椒红色素进行分离操作，然后对分离出的组分进行薄板层析实验。结果表明，提取辣椒红色素使用的最优的溶剂为乙酸乙酯，提取反应时间2。5h。减压蒸馏之后，辣椒红色素的脱辣效果利用感官评定的结果是，上层为呈淡黄色的有机层，下层为透明的的水相。薄板层析最适的展开剂为二氯甲烷，辣椒红色素组份有多达十多个以上。柱层析用二氯甲烷作为洗脱剂，分出五组份，通过极性差异，初步判断为淡黄色为董菜黄素，橙色为β-胡萝卜素，红色为六氢番茄红素，黄色为叶黄素，深红色为辣椒红素和辣椒玉红素。72621

毕业论文关键词： 干辣椒；乙酸乙酯；索氏提取器；薄板层析；柱层析

extraction process of red pigment in chilli pepper

Abstract: Using Sichuan dry chilli as raw material, six different solvents were used to extract red pigment from dried chilli。 The optimum solvent was selected and prepared by thin plate chromatography and column chromatography。 The product was analyzed for the content of the components。 The effects of different solvent extraction conditions and different reaction times on the yield of the product were investigated。 The red pigment was concentrated by vacuum distillation and subjected to deodorization treatment。 In this experiment, four different developing agents were used to analyze the thin layer, analyze the optimum developing agent, and then separate the pepper red pigment by column chromatography。 Then, the isolated components were subjected to thin plate chromatography。 The results showed that the optimal solvent for extracting chili red pigment was ethyl acetate, and the extraction time was 2。5h。 After the vacuum distillation, the hot pepper red pepper effect of the use of sensory evaluation results in the upper layer was pale yellow organic layer, the lower layer is transparent water phase。 Thin plate chromatography is the most suitable agent for the development of dichloromethane, chili red pigment components up to more than a dozen or more。 Column chromatography with dichloromethane as eluent, pided into five components, the initial judgment for the yellow yellow as the yellow vegetables, orange β-carotene, red hexahydron lycopene, yellow lutein, Deep red for capsaicin and chrysanthemum。

Key words:  Dried pepper; ethyl acetate; Soxhlet extractor; thin plate chromatography; column chromatography

目录

1绪论 1

1。1 开题依据 1

1。2文献综述 2

  1。3 方案论证 4

2 实验部分 6

2。1 仪器与试剂 6

2。1。1 仪器 6

2。1。2 试剂 6

2。2实验原理 7

2。2。1辣椒红色素的成份 7

2。2。2溶剂法 7

  2。2。3索氏提取器 7

  3。3实验步骤