植物几丁质酶的生物信息学分析
毕业论文关键词：植物；几丁质酶；生物信息学
Bio-informatics Analysis of Plant Chitinase
Abstract:Chitinase (EC3.2.1.14) is a class that plants, animals, microorganisms can degrade chitin (N- acetylglucosamine linear oligomers) glycosidases. Its resistance to fungi and plants related to defense systems, is an important pathogenesis-related proteins. We queryed and download Triticum aestivum （ AAR11388.1）、Sorghum bicolor（ XP-002488863.1)、Oryza sativa japonica group （NP_001050373.2)、Ricinus communis （XP_002525743.1)、Arabidopsis ( XP_00284907.1)、Zea mays (AAT40016.1) protein sequence of the chitinase from the NCBI database. We are analyzed their basic physical and chemical properties, subcellular localization, transmembrane region, homology comparison and phylogenetic tree, as well as secondary and tertiary structure prediction and a series of bioinformatic analysis to understand its structure and function related information. These results indicate that the signal peptide sequences of these proteins are present, are secreted proteins, located in the cytosol, it contains a number of different phosphorylation sites; chitinase protein sequence length and isoelectric selection of six plants point is insignificant, these types of protein sequences are present disorder zones 5-8, they all belong to globulin, and the sequence of 20 amino acids commonly found close; the secondary structure of the six plants of chitinase are no rules curly-based. Rhodopseudomonas palustriss because they had close evolutionary distance. Seen through the evolutionary tree constructed by constructing evolutionary trees that, these types of higher plant similarity, close evolutionary distance. All results provide a theoretical basis for further research.
Keywords: plant; chitinase; Bioinformatics analysis
目 录
摘 要 1
引 言 2
1 材料和方法 3
1.1材料来源 3
1.2 生物信息学分析软件 4
2 结果与分析 5
2.1生物信息学分析 5
2.1.1 基本理化性质分析 5
2.1.2几丁质酶跨膜区预测 7
2.1.3几丁质酶的信号肽分析 8
2.1.4 几丁质酶的亚细胞定位 8
2.1.5几丁质酶磷酸化分析 9
2.2 几种蛋白序列的同源性比对与同源进化树的构建 9
2.3 二级结构、蛋白紊乱区、球蛋白区预测 11
2.3.1蛋白质二级结构预测 11
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