摘要冬寒菜其营养丰富,应用价值高,有较大的研究意义。本实验为探究光照、温度对冬寒菜种子萌发的影响,以及冬寒菜种子萌发过程中α-淀粉酶活力的动态变化的萌发特性为目的。种子萌发观测指标:计算累积萌发率=所有萌发种子数量占总的供试培养种子数量的百分比。本实验以紫叶冬寒菜和绿叶冬寒菜种子为材料,通过冬寒菜种子的萌发实验,从提高冬寒菜种子发芽率角度出发,研究冬寒菜种子萌发特性,实验内容一方面探讨冬寒菜种子的萌发受温度和光照的影响,另一方面是测定冬寒菜种子萌发过程中连续的α-淀粉酶活力的动态变化,探讨α-淀粉酶活力与种子萌发过程的相关性[1]。实验结果从设定的三个温度梯度和不同光照条件下的冬寒菜的种子萌发情况、幼芽成苗生长情况、萌发前到萌发基本结束后的α-淀粉酶活力动态变化这三个方面进行分析。从而进一步对冬寒菜种子萌发特性进行研究。本次研究将为冬寒菜的栽培提供一定理论依据。70723
毕业论文关键词:冬寒菜 种子萌发 光照 温度 α-淀粉酶活力
Study on the Germination Characteristics of Curly mallow Seeds
Abstract The experiment exploring light, dynamic changes during α- amylase temperature on Curly mallow seed germination, and Curly mallow seed germination. Seed germination observations: Cumulative germination of all seeds germinated seed vigor possession percentage. In this study, purple leaf green leafy vegetables and c Curly mallow seeds as test material, through the Curly mallow seed germination test, increase cold winter dish from the viewpoint of seed germination, Curly mallow experimentally studied Seed germination characteristics, test content on the one hand Discussion on Curly mallow seed germination affected by temperature and light, on the other hand is measured during germination dynamics of α- amylase and explore α- amylase activity and seed germination relationship. The results from the purple leaf vegetables and Curly mallow leafy vegetables germination rate case temperature gradient and set three different lighting conditions, bud growth, culture α- after germination to substantially before the end of the Curly mallow seed germination analysis on the dynamic changes of amylase activity. In this study, further Curly mallow seed germination characteristics will provide a theoretical basis for the cultivation of Curly mallow.
Key Words:Curly mallow seed germination light temperature α- amylase
目 录
摘 要 I
Abstract II
目录 Ⅲ
图清单 4
表清单 4
1 绪论 5
1.1 实验研究的目的与意义 5
1.2 冬寒菜的形态特性研究现状 5
1.3 种子萌发特性研究现状 6
1.4 冬寒菜种子萌发特性研究的主要内容 6
2 实验材料与方法 7
2.1 实验材料 7
2.2 试剂 7
2.3 仪器 7
2.4 实验方法 7
2.4.1 不同温度下冬寒菜种子萌发率 7
2.4.2 黑暗和光照条件下冬寒菜种子萌发率 8
2.4.3 测定冬寒菜种子萌发过程中α-淀粉酶活力的变化过程