摘要白僵菌是目前世界上研究以及应用最多的一种虫生真菌,属于半知菌纲,链孢霉目,链 孢霉科,它同时也是一种被广泛应用的真菌性杀虫剂。白僵菌制剂对人和牲畜无毒,对作 物安全,无污染,对其它虫子有毒害,但对蚕有毒害。白僵菌作为一种微生物杀虫剂具有很 大的潜力,它的杀虫作用会受到环境因素、菌株的影响,同时它也会受到自身的影响。白 僵菌的孢子在接触害虫的时候产生芽管,它是通过皮肤侵入其体内然后长成菌丝,在此期 间不断繁殖导致害虫新陈代谢紊乱而使其死亡。82341

基因决定生物的性状表达,既然白僵菌在作为生物杀虫剂方面具有很大的潜力,白僵 菌是真菌,真菌有线粒体,我在本次实验中是以白僵菌的线粒体 DNA 作为分析对象,白僵 菌对害虫的侵染过程与病毒侵染噬菌体过程类似,在侵染过程中线粒体 DNA 会进入害虫 体内,我利用 DNA 二代测序技术得到白僵菌基因序列,利用相关生物学软件分析,分析其 对家蚕的侵染是否与基因片段相关信息,这个对于研究白僵菌还是很有意义的。。

毕业论文关键词：白僵菌；线粒体 DNA；DNA 二代测序；生物信息学分析

Abstract Beauveria bassiana is currently the world on research and application of the most a kind of insect endophytic fungi, belonging to the fungi imperfecti, moniliales, Neurospora science, it also is a kind of widely used fungal insecticides。 Beauveria bassiana preparation to people and livestock non-toxic, safe to crops, no pollution, the other bugs are toxic, but for silkworm toxic。 Beauveria bassiana as a microbial insecticide has great potential and its insecticidal effect will be influenced by environmental factors, influence of the strains。 At the same time, it will affected by its own。 The spores of Beauveria bassiana  in  pests touch produce germ tubes, it is through the skin to invade the body and grew mycelium, during continuous breeding Causes the insect metabolism disorder and causes it to die。

Genes determine traits of biological expression, since Beauveria bassiana in as a biological insecticide has great potential, Beauveria bassiana fungus, fungal mitochondria,  I in this experiment is in mitochondrial DNA of Beauveria bassiana as the analysis object, Beauveria bassiana on pest infection and virus infecting phage process is similar, in the infection process of mitochondrial DNA will enter the body of insect pest, I use DNA next generation sequencing technologies are gene sequence of Beauveria  bassiana, using biological software analysis, the analysis of the infection of silkworm whether information associated with the gene fragments, the for the study of Beauveria bassiana significance  is still。

Keywords: Beauveria bassiana; mitochondrial DNA; DNA two generation sequencing; bioinformatics analysis

目 录

第一章 绪论 1

1。1  白僵菌概述 1

1。1。1 白僵菌 1

1。1。2 市场价值 2

1。1。3 生物学价值 2

1。2  白僵菌生物学特性及致病性 3

1。2。1 白僵菌生物学特性 3

1。2。2 白僵菌的致病性简介 4

1。2。3 球孢白僵菌的致病机理 6

1。2。4 球孢白僵菌对地下害虫的防治 7

1。3 DNA 二代测序 8

1。3。1 DNA 二代测序简介 8

1。3。2 测序技术发展历程