亚硝酸盐还原酶发酵工艺研究
关键词：亚硝酸钠；乳酸菌；海藻酸钠固定；发酵罐
Study on fermentation technology of nitrite reductase
Abstract: Nitrite, as intermediate product of the nitrogen cycle, is potential carcinogens and detected in most of the food. In recent years, the nitrite content exceed the standard of food, water, aquaculture and the environment, resulting in varying degrees of damage. This topic using lactic acid bacteria produced nitrite reductase, the first study of sodium nitrite added time, through the cultivation of the same time, sodium nitrite and cultured in 6h after adding lactic acid bacteria degradation than directly adding increased 9.12µg/mL, the degradation effect is better. Secondly, in the research of medium nitrogen source, nitrogen source, namely sodium nitrate or ammonium sulfate, the higher the concentration, the degradation effect is better; and the yeast extract the optimum adding amount of 7g, the best time of sodium nitrite degradation effect; when the nitrogen source for sodium nitrate, sodium nitrite degradation of adding lactic acid bacteria in the 2h after the train reached the highest, 96µg/mL as nitrogen source, and ammonium sulfate is added, 8h degradation was the highest, 71.44µg/mL. Finally, the process of sodium alginate was studied; the optimal embedding rate was 4%, and the wastewater bacteria suspension degradation effect increased significantly after 6h, 18h degradation of 17.9µg/mL.
Key Words: Sodium nitrite；Lactic acid bacteria；sodium alginate fixed；Fermentation tank
目 录
毕业设计(论文) 1
1绪论 1
1.1 亚硝酸盐简介 1
1.2 亚硝酸盐的国内外研究现状概述 1
1.2.1 亚硝酸盐在食物中的来源 1
1.2.2 目前国内外降解亚硝酸盐的方法 2
1.2.3 细胞固定化技术 4
1.3 本课题的研究目的和意义 5
1.4 研究内容 5
2材料和方法 6
2.1 材料 6
2.1.1 实验菌种： 6
2.1.2 实验试剂 6
2.1.3 培养基 6
2.1.4 实验设备 7
2.2 实验方法 8
2.2.1 亚硝酸钠的测定：盐酸萘乙二胺法 8
2.2.2 降解亚硝酸钠的测定 9
2.2.3 培养基氮源的研究 10
2.2.4 培养基氮源的优化 10
2.2.5 不同加样时间对降亚硝酸钠的研究 11
2.2.6 菌体海藻酸钠固定化降解亚硝酸钠 11
3结果与分析 13
3.1 降解亚硝酸钠的研究 13
3.1.1 降解亚硝酸钠的测定 13
3.1.2 培养基氮源的研究 14
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