摘要拟南芥（Arabidopsis thaliana）是一种重要的模式植物，也是植物生理学、遗传学研究的重要材料。拟南芥生长素受体基因AtTIR1受到盐胁迫的诱导，其表达产物通过参与转录调控，间接影响了植株的盐胁迫耐受性。黄瓜(Cucumis sativus L。)作为重要的经济作物，其盐胁迫耐受能力非常重要。CsTIR基因是黄瓜中的生长素受体同源基因，其功能尚未有明确的报道。本研究利用黄瓜CsTIR转基因高表达拟南芥作为研究材料，考察转黄瓜CsTIR基因拟南芥对盐胁迫耐受性的影响，并且分析了其潜在机理。本实验用300 mM NaCl处理转基因拟南芥和野生型拟南芥的种子、幼苗与成年植株，统计了转基因种子相较野生型在高盐培养基上的萌发率，观测比较了幼苗生长情况以及测量成年植株抗逆境生理指标。76644

获得的主要实验结果如下：300 mM NaCl处理下，转基因拟南芥种子萌发率和萌发速度相较野生型有一定程度的提高，转基因幼苗侧根数量增加、对叶绿素合成的抑制作用有所减弱。实验发现黄瓜CsTIR基因对拟南芥成年植株叶片SOD活性和丙二醛含量的影响不显著。本研究说明，发现转黄瓜CsTIR基因拟南芥盐胁迫耐受性提高，揭示了黄瓜CsTIR基因的潜在功能，为黄瓜生长素受体的功能研究和其潜在的耐盐机制研究提供新的数据和参考。

毕业论文关键词：拟南芥；黄瓜；CsTIR；盐胁迫；生长素受体基因

Abstract Arabidopsis is an important model plant, as well as an important material for plant physiology and genetic research。 It is reported that auxin receptor AtTIR1 was induced by salt stress, and participated in transcriptional regulation under salt stress, which influence the salt stress tolerance in Arabidopsis。 Cucumber (Cucumis sativus L。) is an important economic crop, and is very important in salt stress tolerance。 CsTIR gene is one of the auxin receptor homologous genes in cucumber, whose function had not been reported yet。 We used CsTIR transgenic plants as materials to explore the potential influence of CsTIR in salt stress tolerance and study its mechanism in Arabidopsis。 In this work, both transgenic CsTIR and wild-type plants were treated with 300 mM NaCl, germination rate of seeds were counted, and the growth of seedings were observed and measured。

The main results are as follows: under 300 mM NaCl, transgenic plants had an enhanced seed germination percentage and rate, increased numbers of lateral roots, and decreased inhibition of chlorophyll synthesis。 The SOD activity and the content of MDA in adult plant leaves showed no obvious difference between transgenic CsTIR and wild-type plants。 This work illustrated that transgene of cucumber CsTIR enhanced salt stress tolerance in arabidopsis thaliana and showed its potential function。 This work also contributes to the further study of their functions and mechanisms in salt stress tolerance in cucumber。

Keyword:  Arabidopsis; Cucumber; CsTIR; Salt stress; Auxin receptor gene 

目    录

引言 5

1 黄瓜CsTIR基因影响拟南芥的盐胁迫耐受性 6

1。1 材料与方法 6

1。1。1 材料 6

1。1。2 主要试剂和主要使用仪器 6

1。1。3 盐胁迫下种子萌发率统计 6

1。1。4 盐胁迫下幼苗根的生长情况统计 6

1。1。5盐胁迫下土培苗生长状态统计 7

1。2 实验结果 8

1。2。1 CsTIR基因影响种子在盐处理下的萌发率